CHEK1 anticorps (AA 1-210)

N° du produit ABIN5518819

L’anticorps Lapin Polyclonal anti-CHEK1 a été validé pour WB et FACS. Il convient pour détecter CHEK1 dans des échantillons de Humain.

Aperçu rapide pour CHEK1 anticorps (AA 1-210) (ABIN5518819)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-CHEK1 anticorps

Épitope: AA 1-210

Fonction: Anti-Chk1/CHEK1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Chk1/CHEK1 Antibody Picoband® (ABIN5518819). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Chk1 recombinant protein (Position: M1-Q210). Human Chk1 shares 96.7% and 97.6% amino acid (aa) sequence identity with mouse and rat Chk1, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Flaggs, G., Plug, A. W., Dunks, K. M., Mundt, K. E., Ford, J. C., Quiggle, M. R. E., Taylor, E. M., Westphal, C. H., Ashley, T., Hoekstra, M. F., Carr, A. M. Atm-dependent interactions of a mammalian Chk1 homolog with meiotic chromosomes. Curr. Biol. 7: 977-986, 1997. 2. Sanchez, Y., Wong, C., Thoma, R. S., Richman, R., Wu, Z., Piwnica-Worms, H., Elledge, S. J. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277: 1497-1501, 1997. 3. Zhang, Y.-W., Otterness, D. M., Chiang, G. G., Xie, W., Liu, Y.-C., Mercurio, F., Abraham, R. T. Genotoxic stress targets human Chk1 for degradation by the ubiquitin-proteasome pathway. Molec. Cell 19: 607-618, 2005.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur CHEK1

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHEK1

Sujet:

Synonyms: Serine/threonine-protein kinase Chk1,2.7.11.1,CHK1 checkpoint homolog,Cell cycle checkpoint kinase,Checkpoint kinase-1,CHEK1,CHK1,

Tissue Specificity: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. .

Background: CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization, the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1 is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Furthermore, CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.

Poids moléculaire: 54 kDa

ID gène: 1111

UniProt: O14757

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN